Fig. 4

IL-33 induces the morphologic change of oligodendrocytes. a Mature OLGs were exposed to 10 ng/ml of IL-33 for 24 h, and then subjected to immunofluorescence (red) using anti-MBP in accompany with DAPI nuclear counterstaining (blue). The interconnected network of OLG processes along with a small cell shape was injured after exposure to IL-33 (arrows). The complex network shape of OLGs in the control culture is indicated by arrowheads. In addition, MBP⁺-OLGs in the cultures treated with vehicle and IL-33 were quantified. The cell size of MBP⁺-OLGs was measured using NIH ImageJ analysis software. b Total proteins were prepared from the cultures treated with vehicle and IL-33. The samples were subjected to western blot analysis for the measurement of MBP. Data are presented as mean ± SEM of the three independent experiments. **p < 0.01, ***p < 0.001 versus the vehicle group. Scale bar in a 50 μm