Fig. 4From: Post-surgical inhibition of phosphatidylinositol 3-kinase attenuates the plantar incision-induced postoperative pain behavior via spinal Akt activation in male micePlantar incision induced a time-dependent increase of spinal pAkt expression in male mice, but not in female mice. The expression of pAkt and Akt was assayed at 0, 0.5, 2, 4, 8, 12, 24 h time-points after plantar incision in male mice (a) or female mice (b). The representative bands (top) for the expression of pAkt in the spinal cord at different time points after plantar incision and the quantitative data (bottom) for the expression of pAkt are shown. The fold change for the density of pAkt was normalized to total Akt for each sample, respectively. The fold change of pAkt in the 0-time point group was set at 1 for quantification. *P < 0.05, **P < 0.01, compared with 0-time point group. (one-way ANOVA, followed by Bonferroni’s multiple comparison test; n = 4 mice in each group). Data are presented as mean ± SEMBack to article page