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Fig. 5 | BMC Neuroscience

Fig. 5

From: Post-surgical inhibition of phosphatidylinositol 3-kinase attenuates the plantar incision-induced postoperative pain behavior via spinal Akt activation in male mice

Fig. 5

Cellular localization of spinal-activated Akt induced plantar incision in male mice. Double immunofluorescence staining of pAkt (red) was performed with cell-specific markers: neuronal nuclei (NEUN, green) for neurons, ionized calcium-binding adapter molecule 1 (IBA1, green) for microglia, and glial fibrillary acidic protein (GFAP, green) for astrocytes at 2 h after plantar incision in male mice. Immunochemistry with pAkt antibody indicated increased pAkt immunoreactivity levels in the spinal dorsal horn (B). Double immunofluorescence staining showed that spinal pAkt was expressed in neurons and microglia (localization with NEUN or IBA1, respectively), but not expressed by astrocytes (no colocalization between pAkt and GFAP). Yellows: colocalization of pAkt with respective cell markers. n = 4 mice in each group. Scale bar = 200 μm (A, B); 50 μm (C–K)

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BMC Neuroscience

ISSN: 1471-2202

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