Fig. 4

The morphology of major organs examined by HE staining and Nissl’s staining after cathodal tDCS. Coordinates of the tDCS site on the skull (a) and the epicranial electrode location (red circle, b) were schematically depicted. The morphology of cortex stained with HE (c) and neurons in cortex and hippocampus stained with Nissl’s staining (d, e). The morphology of heart (f), lung (g), liver (h), kidney (i) and spleen (j). Scale bar = 25 μm. Data were presented as the mean ± SEM. N = 6 rats per group. No significant difference was observed between tDCS and control groups