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Table 1 Primary antibodies used in the study

From: Spatio-temporal characterization of S- and M/L-cone degeneration in the Rd1 mouse model of retinitis pigmentosa

Protein

Source

Clone/cat. no.

Species

Immunogen

Dilution

CD68

Bio-Rad

clone FA-11

Rat

Purified concanavalin A acceptor glycoprotein from P815 cell line

1:500a

Iba1

Wako

019-19741

Rabbit

Synthetic peptide corresponding to C-terminus of Iba1

1:4000a

1:20,000

M/L-opsin

Merck-Millipore

AB5405

Rabbit

Recombinant human red/green opsin

1:1500a

1:5000

Rhodopsin

Abcam

clone RET-P1

Abcam

Membrane preparation from adult rat retina

1:1000

S-opsin

Santa-Cruz

sc-14363

Goat

Peptide mapping at the N-terminus of the opsin protein encoded by OPN1SW of human origin

1:1500a

  1. aDilution used for 2-step fluorescent immunostaining procedure. Wako Pure Chemicals, Osaka, Japan; Bio-Rad, Gladesville, Australia; Santa Cruz Biotechnology, CA, USA; Merck Millipore, Kilsyth, Australia; Abcam, Cambridge, UK; Dako, Carpintería, CA, USA