Fig. 6
From: Inhibition of autophagy triggers melatonin-induced apoptosis in glioblastoma cells
Melatonin-induced autophagy is not dependent on the mTOR signaling pathway. The U87MG (a, b) and A172 (c, d) cells treated with PBS (control), rapamycin (200 nM, a positive control), melatonin (1 mM) and agomelatine (2 μg/ml) for 24 h were analyzed for mTOR activity by Western blotting to determine the levels of total and phospho-mTOR, total and phospho- p85S6k, p70S6K and 4E-BP1