Table 1 Primer pairs used for ChIP analysis and qPCR analysis
From: The Bdnf and Npas4 genes are targets of HDAC3-mediated transcriptional repression
ChIP analysis | ||
|---|---|---|
Gene | Forward | Reverse |
Npas4 ChIP | 5′ AGCCCCTTCTCATCCTTTGC | 5′ CTTCCTTGCTTCCCGGTCTT |
Bdnf ChIP | 5′ CACGGCTCTTCCTAGCACTT | 5′ TGTTGATGAGACCCTTTCCATGT |
cFos ChIP | 5′ AGTCTCATCCCCTGACCCTG | 5′ CCTCAGCTGGCCGCTTTAT |
Foxp1 ChIP | 5′ CTTGGAGGATGTTGCTCTGC | 5′ GAGAGGAGGATTTCCAGAAGTT |
Stat3 ChIP | 5′ GTAAGAGGCTCACGGTCTCG | 5′ AACCGCTGAATTACAGCCCC |
qPCR analysis | ||
|---|---|---|
Transcript | Forward | Reverse |
Bdnf exon I | 5′- CAGGGCAGTTGGACAGTCAT | 5′ TACGCAAACGCCCTCATTCT |
Bdnf exon II | 5′ TTCGGCTCACACTGAGATCG | 5′ CAGTATACCAACCCGGAGCTT |
Bdnf exon III | 5′ TTGGAGGGCTCCTGCTTTCT | 5′ CTGGGCTCAATGAAGCATCCAG |
Bdnf exon IV | 5′ ACTGAAGGCGTGCGAGTATT | 5′ TGGTGGCCGATATGTACTCC |
Bdnf exon V | 5′ AAACCATAACCCCGCACACT | 5′ CTTCCCGCACCACAGAGCTA |
Bdnf exon VI | 5′ GATGAGACCGGGTTCCCTCA | 5′ TTGTTGTCACGCTCCTGGTC |
Bdnf exon VII | 5′ ACTGTCACCTGCTTTCTAGGG | 5′ GAGTTCCGCAGACCCTTTCA |
Bdnf exon VIII | 5′ GTGCTCAGGCTAATCCTCGTT | 5′ CTTTCTCCTGGGATGCACAGT |
Bdnf exon IXA | 5′ ACGGCGTGAACAGAGATCAT | 5′ ACGGTTTCTAAGCAAGTGACG |
Npas4 | 5′ CAGATCAACGCCGAGATTCG | 5′ GACACCCTTGCGAGTGTAGAT |
Actin | 5′ GCCATGTACGTAGCCATCCA | 5′ GGAGCGCGTAACCCTCATAG |