Fig. 1

Successful establishing of HIE model in vivo. a The zea-longa scores in sham and HI groups. N = 20/group. b The comparison of TTC staining between sham and HI groups, and the white staining represented the ischemic area. N = 5/group. c The percentage of infarct area in sham and HI groups. d Tunel assay showed the apoptotic cells in right cortex. Red fluorescence represents the positive tunel staining, and blue is the nucleus staining with DAPI. N = 3/group. e Apoptosis rate in the right cortex, revealed by the tunel/DAPI. HI hypoxic-ischemic, h hour, TTC Triphenyte-trazoliumchloride, tunel terminal deoxynucleotidyl transferasedUtp nick end labeling staining. Data are presented as the mean ± SD. *p < 0.05, **p < 0.01. Scale bar = 100 μm