Table 1 Oligonucleotide primer sequences for quantitative reverse transcriptase polymerase chain reaction
From: Noise exposure alters MMP9 and brevican expression in the rat primary auditory cortex
Gene | Primer sequence (forward) | Primer sequence (reverse) | Annealing temperature (°C) | Product size (bp) | RefSeq number |
|---|---|---|---|---|---|
VGLUT1 | 5′-TTTCTACCTGCTCCTCATCTCC-3′ | 5′-ACACTTCTCCTCGCTCATCT-3′ | 60 | 574 | NM_053859.2 |
VGLUT2 | 5′-CATGGTCAACAACAGCACCATC-3′ | 5′-CTCCCCGATGCTCTCTTCTATG-3′ | 60 | 574 | NM_053427.1 |
VGAT | 5′-GAAGAATCTCAAGGCCGTGTCCAA-3′ | 5′-CACGTAGATGGCCATGAGCAGCGT-3′ | 60 | 580 | NM_031782.1 |
GAPDH | 5′-GCGAGATCCCGCTAACATCA-3′ | 5′-CTCGTGGTTCACACCCATCA-3′ | 60 | 178 | NM_017008.4 |