Fig. 4

Enrichment of myeloid cells from the spleen. a Living cells were included in a polygon gate, whereas the remaining cells (mostly dead and apoptotic cells) were excluded (SSC v FSC). Gated cells (exclusion of the negative population, see Additional file 1: Fig. S3) were visualized with the (b) SIRP α–A647 v CD45RABC–PE channels and the (c) CD3–FITC v NKR-P1–PB450 channels. d–f Scatter plots for isotype controls. g Following the enrichment procedure, all samples were analyzed by flow cytometry to characterize the final cell suspension. The mean CD3–percentage of SIRP-α positive cells were 81.9% ± 1.73 and 86.6% ± 1.12 for exposed and control, respectively, p = 0.04. The fraction of contaminating cells made up 12.1% ± 1.28 and 8.8% ± 0.77 CD45RABC positive cells, 1.6% ± 0.51 and 1.1% ± 0.14 CD3 positive cells and 0.9% ± 0.14 and 0.5% ± 0.07 NKR-P1 positive cells in the final isolated cell pellet from exposed and control animals, respectively. *p < 0.05, Students t-test