Fig. 4
Effect of NBP treatment on putative microvascular formation around the lesion site in a rat model of ICH. Panel (a) are micrographs from the striatal injection sites in the brains of representative animals, with the groups and surviving time points as indicated. Shown are immunolabeling with the CD34 antibody with toluidine blue counterstain. Examples of immunolabeled microvascular profiles are indicated by arrows. Panel (b) plots the means of the total number of CD34 labeled vascular profiles counted over a 20× microscopical field with an area of 0.2 mm2. Panel (c) plots the means of fractional area (%) of CD34 labeled vascular profiles. Results from two-way ANOVA followed by Bonferroni’s post hoc tests are marked with symbols. *: Significantly different comparing to the Sham groups; #: Significantly different comparing to the ICH groups; &: Significantly different comparing to the ICH + NBP10 groups. Scale bar = 100 µm in (a), applying to all image panels