Fig. 2

Representative images of brain sections from piglets that underwent sham surgery or cardiac arrest (CA) injury. a, b Macrophotographs show H&E-stained sections of anterior and posterior putamen and motor cortex where neurons were counted in delineated areas. Brain section orientation is indicated. Scale bar is 5 mm. c H&E-stained putamen in a sham-operated pig that received vehicle treatment. Black arrowheads denote morphologically normal neurons. d H&E-stained putamen in a CA + vehicle piglet shows morphologically normal neurons (black arrowheads) and typical ischemic-necrotic morphology denoted by red arrowheads. The ischemic-necrotic neurons have a shrunken, angular cell body; eosinophilic cytoplasm; and a condensed, eccentrically placed nucleus. e H&E-stained putamen in a sham-operated piglet that received TPPU. Black arrowheads denote morphologically normal neurons and red arrowheads denote injured neurons characterized by a round, condensed, and eccentrically placed nucleus, presence of nucleolus, and eosinophlic cytoplasm. The photos in c, d, and e were taken at ×400 magnification and the scale bar is 50 μm