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Fig. 1 | BMC Neuroscience

Fig. 1

From: SCN11A gene deletion causes sensorineural hearing loss by impairing the ribbon synapses and auditory nerves

Fig. 1

CRISPR/Cas 9-mediated generation of a Nav1.9−/− mouse model. a A representative illustration of the CRISPR/Cas9 targeting strategy for generating Nav1.9 knockout (KO) mice. The Cas9 mRNA and two single guide RNAs targeting a region from SCN11A exon 3 to 5, were microinjected into mouse zygotes. b Schematic diagram of primer pair design for PCR genotyping, a representative PCR genotyping result for Nav1.9 wild-type (WT), homozygous (Nav1.9−/−) and heterozygous (Nav1.9+/−), the region of junction of DSB is absent in the WT mice. Primer 2: primer pairs containing forward primer and KO specific reverse primer; Primer 1: primer pairs containing forward primer and WT specific reverse primer. c This successfully eliminated all of exon 3, 4 and 5, as confirmed by Sanger sequencing, induces reading frame shift and thus a premature translational- termination codon during the truncated protein expression. d The protein expression of Nav1.9 in the cochleas of Nav1.9−/− mice (n = 3) or WT mice (n = 4) was measured by western blot

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