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Fig. 5 | BMC Neuroscience

Fig. 5

From: Ammonia induced microglia activation was associated with limited effects on connexin 43 and aquaporin 4 expression in an astrocyte-microglia co-culture model

Fig. 5

Western blot analysis of AQP4 expression under hyperammonemia. a The AQP4 expression was increased significantly only in M5 astrocyte-microglia co-cultures treated with 10 mM NH4Cl for 6 h. This effect was not shown after incubation with NH4Cl for 24 h (n = 4 independent experiments). b In M30 co-cultures, AQP4 expression was not altered by ammonia. Data were collected by n = 4 independent experiments. Comparisons between more than two groups with normal distribution were analyzed using One-way analysis of variance (One-way ANOVA) and Kruskal–Wallis test, *p < 0.05, **p < 0.01, ***p < 0.001

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