Fig. 10

Testing social interaction and social memory using the three-chamber test. Wild type, 5xFAD, 5xFAD/MMP9KO and MMP9KO mice were tested for A, B social interaction (one mouse vs empty cage) and C, D social memory (novel mouse vs familiar mouse) in the three-chamber test. Time spent in A, C the whole chamber containing the mouse/empty cage and B, D the proximal zone surrounding the cages was measured (areas shown in green in each accompanying schematic). Animals were approximately 6 months of age and values represent mean ± SEM (N = 12, 6 males, 6 females for each group). Statistical significance was determined by ANOVA followed by the BKY procedure. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. WT Wild-type, MMP Matrix metallopeptidase, FAD Familial Alzheimer’s disease, MMP9KO MMP9 knockout, SEM Standard error of the mean, ANOVA Analysis of variance, BKY Benjamini, Krieger, and Yekutieli