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Fig. 2 | BMC Neuroscience

Fig. 2

From: In vivo oxygen measurement in cerebrospinal fluid of pigs to determine physiologic and pathophysiologic oxygen values during CNS infections

Fig. 2

Overview of experimental setup and measurement system to determine physiologic and pathophysiologic parameters in CSF. a Experimental set-up of infection to cause meningitis in pigs. Pigs were infected intravenously with S. suis or mock infected with PBS. After a fixed time-frame of eight-12 h post infection, all pigs were anesthetized with isoflurane for eight h under controlled conditions. A needle was inserted into the CSF compartment and connected to the measurement system shown in b. One, four and seven hours after begin of the anesthesia the measurement and sampling were conducted. Finally, pigs were euthanized, and a necropsy was performed. b The measurement system was connected to the needle. Oxygen and pH sensors were connected to specific read-out systems (OXY-1 ST device and pH-1 mini) and a laptop to collect data. A pressure transducer was connected to the anesthesia multiparameter monitor. Three-way valves allowed directing the flow of the CSF to the sensors, into sampling tubes and to the pressure transducer. Furthermore, a cleaning procedure of the system between the measurement points was possible without contaminating the CSF. Detailed information can be found in Additional file. c During the time of isoflurane anesthesia, the pig was fixed in a lateral position with maximal bent head to the sternum. The intersection point (3) was identified by two lines between points 1 and 2. The position of the needle (1 cm caudal of the intersection point) was never changed during the experiment. d The measurement system connected to the needle inside the subarachnoid space of a pig under anesthesia is shown

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