Fig. 9

Euthyroid HT increases microglial engulfment of synaptic elements in the frontal lobe. a, b Representative images showing immunofluorescence labeling for the microglial marker Iba1 (green) and the synaptic marker PSD95 (red) in the frontal lobe of control (a) and HT (b) mice. The nuclei was stained with DAPI (blue). Insets shows localization of PSD95-labeled puncta (arrow) in microglia at higher magnification. c Representative EM images of synaptic elements (arrowhead) engulfed by microglia (M; blue) in HT mice. d PSD95-labeled puncta per microglia. Data are the mean ± SEM (n = 5 mice /group); Scale bars: a and b = 20 μm; insets = 5 μm; a = 500 nm