Protective effect of transgenic expression of porcine heat shock protein 70 on hypothalamic ischemic and oxidative damage in a mouse model of heatstroke

Table 4 The hypothalamic concentrations of malondialdehyde, oxidative-form glutathione (GSSG), reduced-form glutathione (GSH), and GSSG/GSH ratio obtained from non-heated [-]HSP72 ([-]HSP72-N) mice, non-heated [+]HSP72 ([+]HSP72-N) mice, heated [-]HSP72 ([-]HSP72-H) mice, and heated [+]HSP72 ([+]HSP72-H) mice.

Treatment Groups	Malondialdehyde (n mol/mg protein)	GSSG (pmol/μg prot.)	GSH (pmol/μg prot.)	GSSG/GSH
[-]HSP72-N	4.5 ± 0.3	2.2 ± 0.2	3.9 ± 0.3	0.56 ± 0.15
[+]HSP72-N	4.6 ± 0.3	2.4 ± 0.3	3.8 ± 0.4	0.63 ± 0.22
[-]HSP72-H	9.9 ± 0.8^a	3.6 ± 0.2^a	2.0 ± 0.4^a	1.80 ± 0.31^a
[+]HSP72-H	4.8 ± 0.4^b	2.5 ± 0.3^b	4.2 ± 0.4^b	0.59 ± 0.18^b

Samples were measured 4 h post whole body heating for heated groups or the equivalent time period for the non-heated groups. ^aCompared with non-heated groups (P < 0.05); ^bCompared with [-]HSP72-H group (P < 0.05). Data are means ± S.E.M. of 12 mice per group.

ISSN: 1471-2202