Figure 2

Representative autoradiograms of hypothalamic areas of HIB hamster. Well integral representative transverse sections of hypothalamic areas were used to determine the expression pattern of the α GABA_AR subunits considered in the present study. For this purpose oligonucleotidic antisense for α₁ (A), α₂ (B), α₄ (C), and α₅ (D) mRNAs developed in the brain of HIB Mesocricetus auratus and was compared to the same subunits (A', B', C', D') for NHIB hamsters with respect to (E) nonspecific binding autoradiograms. In this case the nonspecific binding section of HIB proved to be similar to NHIB and so was used for all determinations. MPOA: medial preoptic area; Pe: periventricolar hypothalamic nucleus; Sch: suprachiasmatic nucleus.