Figure 1

Laser microdissection of thoracic propriospinal (TPS) neurons for microarray analysis. A , TPS neurons selected for microdissection were located within laminae V, VII, VIII, and X of the thoracic spinal cord. B , Thoracic propriospinal (TPS) neurons retrogradely labeled by fluorogold (FG) injections into the lumbosacral enlargement are shown at 10 × magnification under an ultraviolet filter. The white dashed line marks the boundary of the white and gray matter, while the yellow dashed line outlines the laminae area from which neurons were collected. C , Region from which a FG labeled TPS had been individually laser microdissected yielding a relatively pure TPS neuron RNA message. Figure 1a was adapted from: The Spinal Cord (Watson C, Paxinos G, Kayalioglu G ed.) p 270, New York, Academic Press