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Table 1 aNS-1 cell culture survival during differentiation in 96-well microplates

From: Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates

Exp #

Passage #

Alive cell (%)

 

DIV3

1

2

2'

4

5

7

18

23

23

32

32

39

81.92 ± 2.86 (n = 3783)

81.29 ± 4.55 (n = 4017)

86.38 ± 2.58 (n = 10425)

n.a.

n.a.

87.20 ± 1.71 (n = 5158)

DIV7

1

2

2'

4

5

7

18

23

23

32

32

39

75.61 ± 2.80 (n = 5166)

74.01 ± 1.05 (n = 4394)

84.28 ± 3.74 (n = 11681)

76.40 ± 2.12 (n = 4457)

81.82 ± 1.38 (n = 4531)

85.70 ± 2.40 (n = 7310)

DIV14

1

2

2'

4

5

7

18

23

23

32

32

39

75.41 ± 3.16 (n = 4000)

66.72 ± 11.79 (n = 3595)

76.67 ± 2.69 (n = 11441)

74.96 ± 1.43 (n = 3757)

71.51 ± 2.32 (n = 3526)

65.01 ± 3.28 (n = 4947)

DIV21

1

2

2'

4

5

7

18

23

23

32

32

39

66.81 ± 2.92 (n = 3423)

74.77 ± 5.32 (n = 3988)

74.91 ± 3.72 (n = 7433)

72.44 ± 3.47 (n = 4187)

66.55 ± 6.19 (n = 3503)

69.66 ± 3.38 (n = 5108)

  1. Data were evaluated by Attovision software. Experiments 1, 2, 4, 5, 7 were performed in 96-well microplates. Experiment 2' was performed in 384-well microplate. Experiment 2 and 2' were run in parallel. aNS-1 cells were maintained in vitro for the indicated number of passages (Passage #); n: number of cells analyzed.