Lipopolysaccharide-induced inflammation attenuates taste progenitor cell proliferation and shortens the life span of taste bud cells

Table 1 RT-PCR primers used for gene expression analysis

Gene Name	GenBank Accession #	Forward Primer	Reverse Primer	Product (bp)
Cytokines:
IFN-γ	NM_008337	AGCAACAGCAAGGCGAAAA	CTGGACCTGTGGGTTGTTGA	71
IL-1β	NM_008361	GTAATGAAAGACGGCACACC	ATTAGAAACAGTCCAGCCCA	270
IL-6	NM_031168	TCATATCTTCAACCAAGAGGTA	CAGTGAGGAATGTCCACAAACTG	230
IL-12	NM_008352	CGAATCCAGCGCAAGAAAGA	GGAACACATGCCCACTTGCT	173
MCP-1	NM_011333	CAGCAGGTGTCCCAAAGAAG	GACCTTAGGGCAGATGCAGT	165
TNF-α	NM_013693	CCTCACACTCAGATCATCTTCTCA	TGGTTGTCTTTGAGATCCATGC	147
Cell cycle markers and regulators:
Ki67	NM_001081117	TCTGATGTTAGGTGTTTGAG	CACTTTTCTGGTAACTTCTTG	177
Cyclin B2	NM_007630	CAACCGTACCAAGTTCATCG	CATACAGGATCTGAGAAGCG	169
E2F1	NM_007891	ACCATCACCTCCCTCCACAT	TGGTGACAGTTGGTCCTCTT	127
Endogenous control:
β-Actin	NM_007393	GATTACTGCTCTGGCTCCTA	ATCGTACTCCTGCTTGCTGA	142

GenBank accession numbers for the sequences used to select the corresponding primers are given. The sizes of the predicted PCR products are indicated in base pairs.

ISSN: 1471-2202