Figure 1
Neurotoxicity induced by GT1b in neuron-enriched mesencephalic cultures. (A,B) Cultures were treated with 20 μg/ml GT1b for 24 h and then immunostained with TH antibody. Note a significant loss of TH-ip neurons in mesencephalic cultures following treatment with GT1b (B), compared with untreated control cultures (A). Most of remaining neurons had the short process (white arrows) and rounded and shrunken cell bodies (black arrows). Scale bar, 50 μm. (C) TH-ip neurons were counted or the levels of [3H]dopamine uptake were measured in cultures treated with 2-60 μg/ml GT1b for 24 h. The results are expressed as a percentage of the control cultures. (D) Reduction in the number of NeuN-ip, GABA-ip and TH-ip neurons in cultures treated with 20 μg/ml GT1b for 24 h. The values represent the mean ± SEM of triplicate cultures in four separate samples; *P < 0.05, **P < 0.001, significantly different from each control cultures (ANOVA and Student-Newman-Keuls analyses).