Figure 3From: An evolutionary conserved region (ECR) in the human dopamine receptor D4 gene supports reporter gene expression in primary cultures derived from the rat cortexSp1 represses the D4ECR1 expression in dissociated cultures of neonate rat frontal cortex. Transcriptional activities of D4ECR1 and control plasmid (pGL3p) were equalled to 100%. Both D4ECR1 and pGL3p plasmids were co-transfected with 2 concentrations of Sp1 expression vector (0.5 and 1 μg). Sp1 significantly down regulated transcriptional activity supported by D4ECR1 (Student's T-test p ≤ 0.01 = **; n = 9, 2 day old neonates). The effect of Sp1 over-expression was expressed as the percentage repression on basal transcriptional activity of D4ECR1 and pGL3p.Back to article page