Figure 5From: Sonic hedgehog (Shh)/Gli modulates the spatial organization of neuroepithelial cell proliferation in the developing chick optic tectumRadial organization and immunocytochemical patterns at the end of DS1. A. Ectopic expression of pShhiEGFP in an electroporated OT (ED4.5); patches of positive NE cell bodies at the ventricular zone (VZ) and of postmitotic neurons at the premigratory zone (PMZ) can be observed. B. Hematoxylin-Eosin staining. Arrowheads point to mNE cells located along ventricular zone; they show PH3 nuclear labeling (C) and Notch reactive cytoplasm (D). Arrows point to neurons in the premigratory zone; they display nuclear NeuroD reactivity (E) and βIIITub reactive perikarya (F). Bar: 10 μm.Back to article page