Skip to main content
Figure 6 | BMC Neuroscience

Figure 6

From: Quantitative changes in intracellular calcium and extracellular-regulated kinase activation measured in parallel in CHO cells stably expressing serotonin (5-HT) 5-HT2A or 5-HT2Creceptors

Figure 6

Effect of plating density on ERK activation in 5-HT 2C R-CHO cells. Cells plated at the indicated densities for 24 hrs were preincubated overnight in serum-free medium, and stimulated with vehicle (Veh, open bars) or 1 μM 5-HT (gray bars) for 5 min. Levels of pERK were determined by plate immunoassay and normalized to cell number by crystal violet staining. Background (control wells without primary antibody) value was subtracted. Graph is representative of three independent experiments. n = 8; ** p < 0.01 vs. vehicle.

Back to article page

BMC Neuroscience

ISSN: 1471-2202

Contact us