Figure 4

Effect of administration MKP-7 siRNA on the phosphorylation state of JNK (Thr183/Tyr185) after 4 or 24 h of reperfusion post-ischemia. Non-coding (NC) siRNA was used as a control. Proteins were extracted and measured using antibodies against p-JNK (Thr183/Tyr185), JNK and MKP-7 A: Determination of p-JNK (Thr183/Tyr185) and MKP-7 levels with and without MKP-7 siRNA after 1 or 4 h of reperfusion following 10 min of ischemia. OD data are presented as means ± SEM (n = 4) and expressed as magnitudes of the alteration versus sham control or control. *P < 0.05, versus sham control; #P < 0.05, comparison between chemically treated 4-h and 24-h reperfusion groups and their vehicle control.