Figure 7

Fluorescence photomicrographs showing Ki-67+ (red) and GFP+ (green) cells in GFP-control and GFP-global lines analysed at 80 weeks post-natal. In GFP-control mice, (A) Ki-67+ cells (arrow) are located along the striatal side of the lateral ventricle and (B) GFP staining shows Drd1a-expressing cells and dense neuropil distributed throughout the striatum. There is no overlap between Ki-67+ (white arrow) and GFP+ (blue arrow) cells (panels C and D). Scale bar represents 150 μm in panels A, B, C, and 50 μm in panel D. In GFP-global line mice, Ki-67+ cells (arrow) are distributed along the striatal side of the lateral ventricle (E) and GFP staining shows Drd1a-expressing cells and neuropil distributed throughout the striatum (F). As for GFP-control mice, no overlap of Ki-67+ (white arrow) and GFP+ (blue arrow) cells (G and H) was observed in GFP-global line mice. S denotes striatum and LV denotes lateral ventricle. Scale bar represents 150μm in panels E, F, G, and 50μm in panel F.