Figure 8

The effects of a Ca²⁺ionophore and phosgene (CG) on intracellular Ca²⁺ changes in differentiated vs. undifferentiated M17 cells. M17 cells were cultured in Transwell inserts without and with RA differentiation to 80% confluency. They were then exposed to 5 μM Ca²⁺ ionophore, A23187 or 0 and 16 ppm CG. Intracellular free Ca²⁺ levels were monitored using Fluo-4 Ca²⁺ indicator assay. (A) Change in intracellular Ca²⁺ following A23187 exposure (N=3); (B) Change in intracellular Ca²⁺ following either 0 ppm (air) or 16 ppm CG exposure for 8 min (N=13). *p<0.05 when compared to treated (A23187 or CG) cells.