P-Rex2 is localized to the synaptic layers of the retina. Intense labeling for P-Rex2 is present in the OPL. Punctate labeling for P-Rex2 is distributed across the OFF and ON sublayers of the IPL. Weak labeling is present in some cell bodies in the position of bipolar cells in the INL. Labeling in the inner and outer segments of the photoreceptors is comparable to the autofluorescence commonly observed in these structures. Nuclei are counterstained with DAPI (blue) to illustrate nuclear layers. OS, outer segment; IS, inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar = 20 μm.