Figure 2

Quantification of SV2 isoforms. A: Quantification of SV2A and SV2B in mouse brain by confocal microscopy at P5, P7 and P10. Quantification by confocal microscopy were perfomed by using Olympus software F10 ASW, allowing to measure the intensity of SV2A and SV2B compared to selected surface. Different regions were selected for these measures: hippocampus, hilus of dentatus gyrus (HDG) and CA1of mice at P5, P7 and P10. Three animals per age were used this quantification. Data are presented as mean +/− SD and were analyzed by ANOVA following a Student’s t test. * p < 0.05; **p < 0.01; ns: not significant. B: Quantification of SV2A, SV2B and SV2C in mouse brain by western blot. Fluorescent western blots analysis of SV2A, SV2B and SV2C proteins were carried out on hippocampus, striatum and olfactory bulb (BO) of mice at P5, P7 and P10. Three animals per age were used. Quantification of these western blots was performed using software Image Master 1D Elite. Data are presented as mean +/− SD and were analyzed by ANOVA following a Student’s t test. * p < 0.05; **p < 0.01; ns: not significant. C: Quantification of SV2A, SV2B and SV2C mRNA in mouse hippocampus. mRNA expression levels were measured by RT-qPCR and normalized to the housekeeping gene β-actin. Data were analyzed by a one-way ANOVA followed by a Bonferroni’s Multiple Comparison Test. Three animals per age were used this quantification. This experiment was repeated once with three new animals per age. * p < 0.05; **p < 0.01; ***p < 0.001; ns: not significant.