Figure 7
From: Neurotrophins regulate ApoER2 proteolysis through activation of the Trk signaling pathway
BDNF induces the proteolytic processing of ApoER2 in cortical neurons. (A) Cortical neurons (DIV 7) were treated with 100 ng/mL BDNF for 30 min. AKT phosphorylation levels shown are a result of TrkB activation by its ligand BDNF. Actin is shown as a loading control. (B) Immunofluorescence detection of ApoER2 in permeabilized cortical neurons from rat embryos using an antibody directed against the cytoplasmic domain of the receptor. (C) Serum-starved neurons were pre-treated with 10 μM DAPT for 1 h and then incubated with 100 ng/mL BDNF for 5 h. ApoER2 was recognized from cell lysates using an antibody directed against its intracellular pro-rich region. α-tubulin is shown as a loading control. (D) The blot levels of ApoER2 CTF were normalized to the loading control α-tubulin and plotted as the average ± SD of three independent experiments. Student’s t-test, *P < 0.05.