Figure 4

Simulated currents containing one, two, three channels, and mean times of each open level. (A) Simulated currents containing one (top), two (middle) and three (bottom) independent channels. We used a two state model (Scheme 1) with α = 250 s^-1, and β = 400 s^-1 to simulate the currents. The simulated currents were sampled at 100 kHz and filtered with a digital filter of 10 kHz. The mean closed and unitary current amplitude set to 0 and 1 pA respectively. We set the standard deviation for closed and open level of 0.1 and 0.3 pA to mimic the large open channel noise of P2X₂ receptors. O_i represents the nth open level for multiple channel currents. (B) The mean times of each open level from 5 s of simulated data.