Figure 1
From: A novel BDNF gene promoter directs expression to skeletal muscle
Agarose Gel Electrophoresis of Restriction Enzyme Digests of 5' RACE Clones. DNA was extracted from 48 clones obtained from 5' RACE of total RNA from 10 days old larvae. The clones were digested with EcoRI and SpeI and the inserts separated from the cloning vector by gel electrophoresis through two-tier 1.2 % agarose gels containing ethidium bromide. The DNA was visualized under UV light. The horizontal arrows mark the cloning vector. The vertical arrows point to HindIII digests of phage lambda.