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Table 1 Characteristics of L-Arg stimulated ion channel activity of the three enrichment steps compared with the same parameters from the native L-Arg stimulated channels as reported by Kumazawa et al.*

From: Biochemical enrichment and biophysical characterization of a taste receptor for L-arginine from the catfish, Ictalurus puntatus

Sample

+Unitary Conductance pS

Concentration of L-Arg, μM

Concentration of D-Arg, μM

Cation-anion selectivity

RCA-I lectin column material

58 ± 5

  

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Sephacryl S300 column material

66 ± 3

1 – 50

10 – 200

Weak cation (PNa/PCl = 2.2)

Ion-exchange column material

73 ± 7

  

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Ion Channels from Taste Tissue

40 – 60 75–100

1 – 200

10 – 200

Cation

  1. * From Kumazawa [31] + Data on unitary conductance reported as Mean ± SD obtained from at least three experiments.