Figure 11
Anterograde degeneration of transected WldS axons eventually continues with complete proximal fragmentation. Confocal composite picture showing six consecutive lengths (from top to bottom in overview) of the proximo-distal course of an individual YFP labelled WldS axon within a peripheral triple heterozygote nerve stump 20 days after transection injury demonstrating a clearer anterograde progression of axon degeneration than in Fig. 10. Note that this isolated axon shows complete break-up (white arrows) with clearly demarcated fragments in its most proximal part among a minority of axonal narrowings (red asterisks) (inset 1). Moving further distal fragmentation accompanied by axonal constrictions becomes gradually weaker (inset 2, 3) while at its most distal end almost no degeneration can be identified (inset 4). YFP fluorescence has been pseudo-coloured yellow with the applied confocal imaging software (Biorad LaserSharp 2000). Scale bar: 500 μm