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Table 3 Quantitative real-time PCR (qPCR) does not support Sema3A-induced changes in DRG observed by microarray.

From: Analysis of gene expression during neurite outgrowth and regeneration

Gene

Gene Title

Max. difference by microarray§

Max. difference by qPCR

p-value

Cntn3

Contactin 3

2.1

2.6

0.34

Col18

Procollagen, type XVIII, alpha 1

1.6

2.5

0.38

Fdps

Farnesyl diphosphate synthetase

1.7

1.4

0.18

Gli2*

GLI-Kruppel family member GLI2

1.5

1.8

0.66

Tubb3

Tubulin, beta 3

1.3†

1.7

0.01

  1. p-value indicates significance of qPCR data for a quadratic model of change over time. Italicized p-values indicate lack of statistical significance.
  2. * Selection of these genes included probesets not part of the matched DRG-SCG dataset, as an additional screen for genes missed by these filters.
  3. § Mock-Sema3A differences observed by microarray were statistically significant (quadratic model; p ≤ 0.05).
  4. † By microarray analysis, the mock-Sema3A differences observed for Tubb3 were not statistically significant.
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BMC Neuroscience

ISSN: 1471-2202

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