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Figure 3 | BMC Neuroscience

Figure 3

From: Regulation of nestin expression by thrombin and cell density in cultures of bone mesenchymal stem cells and radial glial cells

Figure 3

Expression of the PAR1 thrombin receptor in RG and rat MSCs cultures. RG and rat MSCs were cultured for 2 days in the presence of serum (A, B, E) or were maintained for the same time in serum-free medium (C, D, F). Then, the cells were fixed and RG were labelled with anti-nestin (green), anti-PAR1 (red) and anti-GFAP (blue) antibody (panel A-D) while rat MSCs were stained with anti-nestin (green) and anti-PAR1 (red) antibody (panel E, F). Scale bar (in A = 10 μm) applies to all images. Expression of thrombin receptor PAR1mRNA in mouse RG (G) and rat MSCs and astrocytes (H) was analyzed by RT-PCR using optimized primers for each species.

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BMC Neuroscience

ISSN: 1471-2202

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