Figure 4

sGC mutants disconnect exploration from detection of odour. (1) Same experiment than in figure 3 except that experiments were carried out in odour-saturated atmosphere. In tube (C), homozygous mutants versus their genetic background give: T value 3.87, P = 0.001, degrees of freedom: 18 (GC253); T value 7.6, P = 0.0001, degrees of freedom: 18 (GC207); T value 4.36, P = 0.0004, degrees of freedom 18 (GC782). The heterozygous mutants show a similar trend (n = 10). Statistics for mutants versus genetic background in tube C: T value 4, P = 0.001, degrees of freedom: 16 (GC253 and GC207); T value 1.98, P = 0.06, degrees of freedom: 16 (GC782). (2) The experiment was also performed using a derivative protocol: one single pierced transparent tube was used with 1 ml grape juice in a saturated atmosphere. We observed progression of the accumulation from 2 to 24 hours. Values are mean ± SEM (n = 10). For GC253bv versus bv at 24 hours: T value 1.8, P value: 0.085, degrees of freedom: 18 (3) Analysis of the GC207 mutant and the rescue hsp-sGC, GC207 (protocol as in (1)). The rescue (hsp-sGC, GC207), along with GC207, exposed to single acute heat shock at late third instar larva plus 3 day old adult stage, showed reverse phenotype (similar to control genetic background). Statistics for the mutant versus the rescue in tube C: T value 3.42, P = 0.003, degrees of freedom: 18 (n = 10).