Table 1 Antibodies used for immunotagging of neuronal nuclei.
Primary/secondary antibody conjugate | |||||
|---|---|---|---|---|---|
anti-NeuN (Ms) | anti-Ms IgG Alexa 488 | Blocking Solution | 1 × PBS | Nuclei solution | |
NeuN | 1.2 μl | 1 μl | 100 μl | 300 μl | 1000 μl |
Negative control | 0 μl | 0.2 μl | 20 μl | 60 μl | 200 μl |