Figure 6

A-C: Higher magnifications of the protocerebrum and median deutocerebrum. Horizontal vibratome sections double labeled for serotonin immunoreactivity (5HT; green) and glutamine synthetase-like immunoreactivity (GS; red); confocal laser scan microscopy. The boxed area in A is shown in a higher magnification in C. The neuropils surrounded by GSir cells display strong immunolabelling in the neuropil core. The bi- or tripolar GSir cells, presumably ensheathing glia cells, extend processes into the neuropil. The arrow in A identifies a large serotonergic neuron. Many more serotonergic somata are located in cell cluster (9). D1, D2: Double labeled sections showing synapsin immunoreactivity (SYN; red) and RFamide-like immunoreactivity (RF; green); confocal laser scan microscopy. In D2, only the green channel is visualized at a slightly higher magnification to show the central body (CB). The arrowheads in D1 identify peptidergic cell somata. E, F: The protocerebral bridge (PB) displays synapsin immunoreactivity (E) and serotonin immunoreactivity (F; confocal laser scan microscopy). The arrowheads identify cell somata within cell cluster (6). The X labels a synaptic region in the chiasm of the olfactory globular tract. Abbreviations: 9 cell cluster (9), AcN accessory lobe/neuropil, AMPN anterior medial protocerebral neuropil, AnN antenna 2 neuropil, LAN lateral antenna 1 neuropil, MAN median antenna 1 neuropil, OGT olfactory globular tract, ON olfactory lobe/neuropil, PC protocerebrum, PMPN posterior medial protocerebral neuropil, PT protocerebral tract.