Figure 4
From: Defects in the retina of Niemann-pick type C 1 mutant mice
Defective in horizontal cells in the Npc1 -/-retina. A-F: Retinal sections from P65 Npc1+/+(A-C) and Npc1-/-(D-F) mice were immunostained with an antibody against calbindin (red). Nuclei were stained with DAPI (blue). B, E show magnification of the ONL from A, D, and C, F of the inner part of the retina from A, D, respectively. G-I: double immunostaining with antibodies against calbindin (red in G) and SMI32 (green in H) revealed ectopically extended axonal terminal-like structures of the Npc1-/- horizontal cells (yellow in I). Abbreviations: ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer; S1-S5, five parallel sublaminae of INL. Scale bar: 10 μm in B for B, E, G-I and in C for C and F; 50 μm in A for A and D.