Figure 2From: Expression, sorting and transport studies for the orphan carrier SLC10A4 in neuronal and non-neuronal cell lines and in Xenopus laevis oocytesTransport measurements in transiently transfected HEK293 cells. HEK293 cells were transiently transfected with the indicated carriers SLC10A4, DAT, CHT1, or SERT, respectively. The uptake of 5 µM [3H]dopamine, 5 µM [3H]norepinephrine, 5 µM [3H]choline, or 5 µM [3H]serotonin was measured over the given time periods in the presence and absence of Na+ (for CHT1 Na+ was replaced by Li+). Transport via DAT, CHT1, and SERT was blocked by the specific inhibitors nomifensine (10 µM), hemicholinium-3 (HC-3, 1 µM), and citalopram (2 µM), respectively. Values represent mean ± SD of representative experiments, each with quadruplicate determinations (n = 4). *Significantly different from control with p < 0.001. #Significantly different from positive uptake, p < 0.001.Back to article page