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Figure 1 | BMC Neuroscience

Figure 1

From: NKCC1 downregulation induces hyperpolarizing shift of GABA responsiveness at near term fetal stages in rat cultured dorsal root ganglion neurons

Figure 1

Intracellular [Cl−] and bumetanide-induced Cl− reduction in DRGN. The resting [Cl−]i (open bars) dropped significantly between E14 (44 ± 2 mM, n = 71) and E16 (30 ± 2 mM, n = 11, p = 0.00003), with no further decrease at E19 (29 ± 1 mM, n = 13, p = 0.81). In the presence of 10 μM bumetanide (+Bumet, grey bars), a specific blocker of NKCC1, [Cl−]i was decreased to 20 ± 1 mM (n = 6) at E14 and to 18 ± 1 mM (n = 6) at E16 and E19. The bumetanide-insensitive component remained stable at all stages (p = 0.73 for E14 versus E16 and p = 0.46 for E14 versus E19).

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BMC Neuroscience

ISSN: 1471-2202

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