Fig. 3
From: High-fat diet-induced obesity exacerbates kainic acid-induced hippocampal cell death
Effects of a HFD on hippocalcin and calpain1 expression KA-treated hippocampus. a Representative microphotographs of double immunofluorescence of hippocalcin and NeuN in the CA3 region of ND, ND + KA, HFD, and HFD + KA mice. Scale bar 50 μm. b Western blot analysis of calpain1. Densitometry values for each protein were normalized to α-tubulin and expressed as fold change relative to the ND group. Data are shown as mean ± SEM (n = 4 mice per group). *p < 0.05 versus ND. # p < 0.05 versus HFD. † p < 0.05 versus ND + KA