Fig. 6

TRKAζII-V5-His is a glycoprotein not targeted to the plasma membrane. HEK293 cells were transfected with plasmids encoding proteins TRKAII-V5-His, TRKAγII-V5-His and TRKAζII-V5-His. The proteins were detected using western blotting and an antibody against the V5-tag. a TRKAζII-V5-His is a glycoprotein. Cells were cultured with (+) or without (−) tunicamycin (Tm, 2 µg/ml), the inhibitor of N-linked glycosylation. Untransfected cells were used as a negative control (neg). b Intracellular localization pattern of tagged TRKA isoforms detected with immunocytochemical analysis. DNA was stained with DAPI, plasma membrane was visualized with Alexa Fluor 488-conjugated concanavalin A. Scale bar 5 µm. c The first 52 N-terminal amino acid residues of TRKAII, TRKAγII and TRKAζII. Amino acids that are considered to be more hydrophobic are in green, less hydrophobic are grey, amino acid residues with positive charges are underlined