Fig. 2

PICALM (a–c), clathrin (d–f), APP (g–i) and BACE1 (j–l) localisation after different siRNA treatments. H4 cells were incubated for 48 h with control siRNA (GFP siRNA, a, d, g, j), siRNA targeting PICALM (PICALM siRNA, b, e, h, k) or clathrin heavy chain (Clathrin siRNA, c, f, i, l). Proteins were detected using a biotinylated secondary antibody and an FITC-conjugated avidin construct (green), nuclei are counterstained with DAPI (blue). Images were taken with a Leica SP5 confocal laser scanning microscope and are representative from n = 3 (scale bar 25 μm). Arrows indicate the reduced cytoplasmic and membrane-associated staining seen after treatment with PICALM siRNA (a) compared to GFP siRNA (b)