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Fig. 7 | BMC Neuroscience

Fig. 7

From: Identification of environmental stressors and validation of light preference as a measure of anxiety in larval zebrafish

Fig. 7

Physiological effects of various stressors and effect of anxiolytic drugs on non-stressed and stressed larvae (a) Relative cortisol level (absolute cortisol concentration/total protein concentration). Data are presented as mean ± SEM; **p < 0.01, *p < 0.05, ns no significance; post hoc Dunnett’s Multiple Comparison test after one way ANOVA compared to control (unstressed) group. Effect of chlordiazepoxide (Chlor) and Buspirone (Busp) on light/dark choice index of non-stressed (b) and heat stressed (HS) (c) larvae. Data are presented as scatter plots showing individual sample values and error bars representing mean and 95 % CI. *p < 0.05, **p < 0.01, ***p < 0.001; n = 13 for control and heat, n = 17 for all other groups, post hoc Dunnett’s Multiple Comparison test after one way ANOVA compared to control (no drug) group

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