Fig. 3

RNA Quality and Reverse Transcriptase-PCR (RT-PCR). a RNA quality. Electropherogram of the RNA sample (3000–4000 pg/µl) from GFP⁺ sorted cone photoreceptors using the Bioanalyzer Pico-Assay. In the final experiment, after the optimization of the techniques, high-quality RNA was obtained with the typical shape of the two ribosomal peaks (18S and 28S), and with RNA Integrity Number of 7.6, which was slightly higher than the minimum-required (7.0) for microarray analysis. b Reverse Transcriptase-PCR (RT-PCR) carried out for actb, egfp, rho, gnat2, pde6h, opn1lw2 and pde6c in GFP-positive cone photoreceptors and GFP-negative retinas. M is NEB 2 Log DNA ladder (0.1–10.0 kb)