Fig. 7
From: Expression of alternatively spliced variants of the Dclk1 gene is regulated by psychotropic drugs
LC–MS/MS detection of CARP/DCLK1-M-derived peptide. a A schematic representation of the Carp and Dclk1-m transcripts and their putative peptide products. Colored boxes mark exons. Peptides were digested with the endoproteinase LysC, which cleaves peptide bonds at the carboxyl side of lysine residues (marked red). The fragment corresponding to the SPSPSPTSPGSLRK peptide is marked with a box. b Detection of the SPSPSPTSPGSLRK peptide. The panels show (from the left): signal for potential contamination with light peptide checked in heavy peptide sample, signal for heavy-labeled peptide spiked into the experimental sample, signal for endogenous (light) peptide in a sample derived from the murine striatum. Colored peaks correspond to peptide fragment ions. Please note the different scales on the y-axes